Composite

Part:BBa_K2066023

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-08-29)


pTet GFP + UNS

This part was created to place the pTet-GFP reporter construct BBa_K1493504 on the UNS backbone standard developed by William and Mary iGEM 2016. It is flanked by UNS 2 (BBa_K2066018) and UNS 3 (BBa_K2066019) sequences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 764


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Categories
Parameters
None